Macrophages have been known to produce monokines and to involve to the cytotoxic activity against microorganisms or tumor cells.
Using murine peritoneal macrophages the effect of interferon-γ(γIFN), lipopolysaccharide (LPS), 1,25 dihydroxy-vitamin D3(DHVD3) or dexamethasone alone or in combination was assessed on the expression of interleukin-1 and tumor necrosis factor,
the
production of nitric oxide(NO)and the tumoricidal activity against lymphocytic leukemic cell L1210.
@ES The results were as follows;
@EN 1) The activity of interleukin-1 was increased in the murine peritoneal macrophages treated with combination of γIFN and LPS, compared with control cells and the cells treated with each agent alone, and decreased by adding DHVD3 or
dexamethasone.
2) The activity of tumor necrosis factor in the combined γIFN and LPS-treated cells was increased in comparison with the cells treated with each agent alone. It was not affected in the combined γIFN and LPS-treated cells by adding DHVD3, while
being
considerably decreased by adding dexamethasone.
3) The expression of tumor necrosis factor mRNA was significantly increased in the combined γIFN and LPS-treated cells. By the addition of DHVD3 the expression was a little decreased, while being considerably decreased by adding dexamethasone.
4) The production of NO was significantly increased in the cells treated with combination of γIFN and LPS, compared with the cells treated with each agent alone, By the addition of DHVD3 the production amount of NO in the combined γIFN and
LPS-treated
cells wasn't changed, however by the addition of dexamethasone the production was suppressed.
5) The tumoricidal activity of cells was significantly increased in the γIFN and LPS-treated cells, compared with the single agent-treated cells. This increased activity wasn't changed by the addition of DHVD3, however, suppressed by the
addition
of
dexamethasone.
These results indicate that DHVD3 slightly affects the expression of cytokine genes and the tumoricidal activity against murine lymphocytic leukemic cell L1210 in murine peritoneal macrophages, while dexamethasone considerably suppresses them.
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